Bacteriological studies on P. multocida and Mannheimia hemolytica isolated from chickens, ducks and turkeys in Beni Suef Governorate with special identification of P. multocida by PCR assay.

摘要

Bacteriological examination of 370 samples which were taken from organs of freshly dead and diseased chickens, ducks and turkeys showing diarrhoea and respiratory disorders (collected from different farms at Beni Suef Governorate) revealed 34 (21.25%), 38 (29.23%) and 14 (17.50%) isolates of Pasteurella multocida, respectively, and 19 isolates of Mannheimia haemolytica, of which 16 (10%) were from chickens and 3 (3.75%) were from turkeys. The biological characters of both P. multocida and M. haemolytica isolates were discussed. A PCR-based technique was used in the identification of P. multocida. All the examined isolates gave a single amplified product of the expected size (460 bp). The antibiogram study showed that P. multocida and M. haemolytica isolated from chickens, ducks and turkeys were sensitive to ceftiofur, gentamicin, lincomycin + spectinomycin and norfloxacin. The results indicated that the recent development of species-specific PCR assay for P. multocida provided a rapid, sensitive and specific method for the identification of P. multocida isolated from chickens, ducks and turkeys.