Lagerstroemia speciosa (L) Pers., ethanolic leaves extract attenuates dapsone-induced liver inflammation in rats

摘要

Drug-induced liver injury is a common cause of acute liver failure. Dapsone is increasingly used in combination with rifampicin for the treatment of leprosy and also for several dermatological disorders. Clinically, abnormal liver function and focal bile duct destruction were reported after dapsone therapy. Lagerstroemia speciosa Pers., commonly known as Banaba has been traditionally used to treat various ailments including diabetes and obesity due to its antioxidant and anti-inflammatory efficacies. This study investigated the hepatoprotective effect of ethanolic banaba leaves extract (EBLE) against dap-sone-induced hepatotoxicity in rats. Dapsone (30mg/kg, i.p.) was administered twice daily for 30days. In separate groups, rats were post-treated orally with EBLE (250 and 500mg/kg) and silymarin (100mg/ kg) once daily for 30days after dapsone administration. The marker enzymes of hepatotoxicity, oxidative stress markers, inflammatory markers and histopathology of liver were done. HPTLC analysis confirmed the presence of 12.87μg of corosolic acid per mg of EBLE. Dapsone administration-induced significant (p< 0.001) elevation of marker enzymes of hepatotoxicity in serum. This treatment also increased lipid peroxidation (p< 0.001) and pro-inflammatory markers (tumor necrosis factor-alpha, transforming growth factor-beta, and nuclear factor kappa-B) expressions (p< 0.001) and decreased antioxidants (p< 0.001) such superoxide dismutase, catalase and glutathione in the liver tissue. All these abnormalities were significantly (p< 0.001) mitigated after EBLE (500mg/kg) and silymarin post-treatments. The results of this study suggest that silymarin and EBLE can be used for dapsone-induced hepatotoxicity.