DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF TRANS-RESVERATROL IN ETHOSOMES

摘要

The present work describes a simple, accurate and validated UV spectrophotometric method for determination of trans-resveratrol loaded in ethosomal formulation. The method was validated for different parameters like linearity, precision, specificity, accuracy, limit of detection (LOD), limit of quantitation (LOQ) and robustness as per ICH guidelines. The wavelength maximum of resveratrol in phosphate buffer pH 7.4 was found to be at 307 nm. The method was found to be linear in the range of 8 to 38 ug mL-1 with a correlation coefficient (r2) of 0.9957. The accuracy of the method was studied by recovery study and % recovery was found to be in the range of 99.24 to 100.14 %. The method is simple, accurate and requires relatively inexpensive instruments. The proposed method can be successfully used for determination of trans-resveratrol loaded into ethosomes.