Cold Atmospheric Plasma Prevents Wrinkle Formation via an Antiaging Process

摘要

Cold atmospheric plasma is a material that generates free radicals through the ionization of air. Despite the application of this material in various medical fields, the precise mechanism underlying its effect at the cellular level is not fully understood. To date, it is known to stimulate the expression of antioxidant transcription factors such as nuclear factor erythroid 2-related factor 2 (Nrf2), forkhead box class O 3a (FOXO3a), and heme oxygenase 1 (HO-1). In this study, we aimed to assess whether and how cold atmospheric plasma affects damaged cells, particularly after exposure to UV radiation. We found that it can promote mitochondrial health by controlling the stability of the membrane potential of mitochondria against UV radiation. Moreover, cold atmospheric plasma prevents senescence in fibroblasts exposed to UV radiation through the upregulation of p53. It also exhibits hormetic effects (i.e., beneficial effects at low doses) on MMP and collagen in senescent fibroblasts exposed to UV radiation. In conclusion, cold atmospheric plasma treatment not only protects fibroblasts from free radicals but also represses the aging of these cells in a UV-radiation-induced aging process.