Sixteen dithiolthiones including oltipraz (5-(2-Pyrazinyl)-4-methyl-1-1, 2 dithiol-3-thione) and anethol dithiolthione (P-methoxyphenyl-1,2-dithiol-3-thione) have been studied using an isocratic high performance liquid chromatographic system. The retention characteristics of these compounds were determined using a conventional C 18 reverse phase column and a μ Bondapack phenyl column. The mobile phases used consisted of several concentrations of methanol and water. All compounds studied were adequately detected in the nanogram range (30–100 ng on column) using ultra violet detection (UV) set at 300 nm. Several analogs and isomers were separated. The retention characteristics of the 16 compounds studied are reported for both columns using 4 mobile phases and chromatograms of reference standards are presented.
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