Cell kinetic analysis of non‐Hodgkin's lymphomas usingin vivoiododeoxyuridine incorporation and flow cytometry

摘要

AbstractThe aim of this study was to analyse dynamic cell proliferation parameters in non‐Hodgkin's lymphomas. Sixty‐one patients with newly diagnosed or with recurrent disease were given iododeoxyuridine (IdUrd) intravenously near 4 h prior to tumour biopsy. After staining with an IdUrd reactive antibody and propidium iodide, S‐phase fraction (SPF), labelling index (LI), S‐phase duration time (Ts) and potential tumour doubling time (Tpot) were determined by flow cytometry. Thirty‐eight samples, 15 low grade (LGM) and 23 high grade (HGM) malignant lymphomas, were possible to evaluate. Twenty‐three cases were excluded due to aneuploidy, insufficient amount of material or technical problems.Tpot values varied between 0.8–32.9 days (mean 7.0 days). HGM lymphomas had shorter meanTpot times than LGM lymphomas (4.8 versus 10.4 days,p=0.05). ForTs the range was 4.2–20.1 h (mean 9.1 h), and a difference between the two histological groups was demonstrated with a longer meanTs for HGM compared with LGM cases (10.0 versus 7.8 h,p=0.04).Tpot showed a negative correlation with SPF (P=0.003), andTs demonstrated a positive correlation to SPF (p=0.02). The clinical significance of the dynamic cell proliferation parameters studied remains to be clarified, but the interelationships betweenTs/SPF andTs/morphologic subtype might be factors of interest for future prognostic studies in mali