Occurrence of premature chromosome condensation in mouse spermatogonia treated with busulfan

摘要

Busulfan induction of premature chromosome condensation (PCC) was examined in mouse spermatogonia. Adult (C3H × SWV) Ft male mice were injected intraperitoneally with busulfan at 10 or 30 mg/kg of body weight and killed 18–72 h later. Polyploid-like mitoses were frequent (ca. ¼ of all sper-matogonial mitoses examined) in both the untreated and treated groups. Most of these were considered to have been derived from normal spermatogonia. In busulfan-treated mice, polyploid-like mitoses with PCC were seen. The frequency of PCC-containing mitoses increased with increasing dose and exposure time. A possible interpretation for PCC induction in mouse spermatogonia is discus