Chemical composition of lipopolysaccharide from an avian strain ofEscherichia coliO18

摘要

Lipopolysaccharide (LPS) was isolated from an avian strain ofEscherichia coliO18 by extraction of the cells with NaCl followed by treatment with phenol–water, methanol precipitation, and gel permeation chromatography. The LPS consisted of carbohydrate, lipid, and a small amount of protein and phosphate. Further analysis showed the presence of glucose, galactose, rhamnose, glucosamine, N-acetylglucosamine, heptose, 2-keto-3-deoxyoctonate (KDO), and ethanolamine. The composition of the LPS is qualitatively similar to that found for LPS obtained from various other bacteria.