Nano-Bio Selenium Synthesized by Bacillus subtilis Modulates Broiler Performance, Intestinal Morphology and Microbiota, and Expression of Tight Junction’s Proteins

摘要

Abstract A green and ecofriendly bio-based synthesis of nano selenium particles was performed using the Bacillus subtilis and the products were characterized by field emission scanning electron microscope (FESEM), dynamic light scattering (DLS) and transmission electron microscopy (TEM) methods. Dietary treatments included a control diet nonsupplemented with selenium and control diet supplemented with different sources of selenium (sodium selenite, organic Se, and nano-bio Se), resulting in a total of 4 treatments with 6 replicates of 10 chicks. Broilers were assessed for performance measures, ileum morphometry, and microbial population and jejunum tight junction proteins’ relative expression. The particle size of the synthesized selenium nanoparticles ranges 40 to 150?nm, with crystalline spherical shape. Inclusion of selenium increased body weight (BW) and improved FCR compared to the control diet (P?