Serological assay of Tobacco streak virus (TSV) in the different plant parts of germplasm and advance generations of Gossypium barbadense

摘要

Tobacco streak virus (TSV), the causal agent of cotton necrosis, is an emerging disease of national significance in India. Tobacco streak virus (TSV), inciting cotton necrosis, exhibits multifarious symptoms which includes purplish brown, necrotic lesions in the leaves, squares, and petioles. Development of diagnostic tools with rapidity will have immense role to play in detection and management of the emerging virus. A simple and rapid procedure of enzyme immunoassay (ELISA) was used to detect and identify viruses in individual plants. Serological assay by double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA) revealed the presence of TSV in leaf, square, stem, root, petiole and pollen grains of germplasm and advance generations ofGossypium barbadense.