Growth compensation and faster development of Colorado potato beetle (Coleoptera: Chrysomelidae) feeding on potato foliage expressing oryzacystatin I.

摘要

Feeding, growth, development and food conversion efficiency of larvae of Leptinotarsa decemlineata reared on foliage from a 'Kennebec' potato line expressing oryzacystatin I (OCI) at about 1% of its total soluble proteins were compared to those of larvae feeding on untransformed foliage from the same line. During stages L1 to L3, larvae feeding on OCI consumed leaf material 14% faster, gained weight 28% faster, and weighed 20% more at the end of the L3 stage, compared to controls. Continued exceptional performance on OCI during the final L4 stage was expressed as faster development than controls, an effect that persisted during pupal development and resulted in emergence of similar weight adults 1 day earlier than controls. Larvae initially maintained on control foliage and switched to OCI foliage during L4 did not overcompensate as those on OCI foliage throughout development, but performed similarly to larvae on control foliage throughout. Total azocaseinase activity in midgut extracts from these 4th instars 1 day after switching to OCI foliage was sensitive to inhibition by a recombinant form of OCI expressed in Escherichia coli, but was no longer sensitive 4 days after switching, indicating a gradual adaptation of the insect digestive proteinase system, based on the production of OCI insensitive proteinases. Despite OCI potato foliage being consumed faster by small larvae using it for food, there was no indication that it was less efficient than untransformed foliage as food protein.