Simultaneous analysis of two urinary biomarkers of oxidative damage to DNA and RNA based on packed-fiber solid phase extraction coupled with high-performance liquid chromatography

摘要

The determination of the concentrations of urinary biomarkers of oxidative damage to DNA and RNA is difficult due to the low content of targets and the complex matrix of urine. A method using polystyrene/polypyrrole (PS/ PPY) electronspun nanofibers as the adsorbent was introduced to the routine urinary treatment and determination of 8-OHdG and 8-oxoG for the first time. And 2-aminoethyl diphenylborate (DPBA) solution was creatively used in the loading and rinsing steps in order to promote the retention of the analytes as well as remove impurities. Under optimal conditions, 8-OHdG, 8-oxoG and IS were separated very well and exhibited a good linearity in the range of 0.5-50 ng mL(-1), with correlation coefficients of R2 > 0.996. Limits of detection (LOD) were 0.058 ng mL(-1) and 0.093 ng mL(-1), and limits of quantification (LOQ) were 0.195 ng mL(-1) and 0.309 ng mL(-1), respectively. The recoveries were 88.8-104.9%. The proposed method was so simple and economical that it had the potential to be applied to batch quantitative analysis of 8-OHdG and 8-oxoG in urine. And it was successfully applied to real urine samples of cancer patients.