Microsatellite diversity analysis and QTL identification among progenies derived from aerobic x basmati rice (Oryza saliva) cross under direct-seeded conditions

摘要

The present investigation was designed to identify QTL associated with various traits under aerobic condition using F-3 and F-4 population derived from the cross MASARB25 (aerobic rice) and IB370 (basmati rice). The phenotyping was done in both field and net house conditions during the kharifseasons of 2013-14 and 2014-15. The result indicated high variation among the population for studied traits and parabolic frequency distribution was recorded for panicle length, effective number of tillers/plant, 1000-grain weight while, for grain length/breadth ratio and root thickness, frequency distribution curve were skewed toward MASARB25. Composite interval mapping identified total 16 QTLs on chromosomes 1, 2, 3, 4, 6, 9, 10 and 12 during both the years. Maximum QTL were detected for grain len gthbreadth ratio. LOD score of these QTT,s ranged from 2.88 (qENT12.1) to 5.51 (qI,B3.1) and explained 61.63% and 69.04% variance, respectively. The QTL mapped for grain yield/plant (qGYP6.1) on chromosome 6 had LOD score of 2.90 and explained 28.4% phenotypic variation. The identified QTL in present investigation showed high phenotypic variation, hence after validation these QTLs could be used for the improvement of rice under aerobic condition.