Antioxidants in combination with omega-3 fatty acids protect rat neonatal cardiomyocytes against H_2O_2-induced oxidative stress

摘要

Objective Oxidative stress is involved in the development of cardiovascular injuries. The aim of the current study was to determine whether pretreat-ment with a combination of antioxidants and omega-3 fatty acids protects rat neonatal cardiomyocytes against H202-induced oxidative stress. Methods Rat neonatal cardiomyocytes were cultured for 2 h with either olive oil to serve as a control or a combination of antioxidants and omega-3 fatty acids (astaxanthin, lutein/zeaxanthin, vitamin C, vitamin E, vitamin D and krill oil) before oxidative stress was induced by H202 for 24 h. Investigations included analysis of reliable markers for oxidative stress.Results Pretreatment of rat neonatal cardiomyocytes with a combination of antioxidants and omega-3 fatty acids resulted in a reduction in induced lipid peroxidation measured by 8-iso-prostaglandin F2al-pha (8-iso-PGF2ct) and malondialdehyde (MDA) levels.In addition, protein oxidation was reduced after calculating the protein carbonyl level.Conclusion These findings suggest that a combination of antioxidants and omega-3 fatty acids may represent potential protection against oxidative stress.