The salt-tolerant phenazine-1-carboxamide-producing bacterium Pseudomonas aeruginosa NF011 isolated from wheat rhizosphere soil in dry farmland with antagonism against Fusarium graminearum

摘要

Fusarium head blight (FHB) disease caused by Fusarium graminearum (Fg) seriously affects the yield and quality of wheat. In this study, after bacterial community analysis of two wheat rhizosphere soils, the genus Pseudomonas was shown to be enriched in normal dry farmland (maize-wheat rotation) compared to that observed nearby paddy farmland (rice-wheat rotation) with serious FHB disease. Subsequently, a P. aeruginosa strain, NF011 with the highest antagonistic activity against Fg and excellent tolerance to 8.0 % of NaCl was isolated from the wheat rhizosphere soil in the normal dry farmland. Dual culture assay results showed that NF011 is a broad-spectrum fungicide for controlling six wheat pathogenic fungi. The major antifungal compound produced by NF011 was identified as phenazine-1-carboxamide (PCN) by LC-MS and nuclear magnetic resonance. 1.0 x 108 CFU/mL of NF011 or 32 mg/L of PCN could completely inhibit Fg spore germination and resulted in the destruction of Fg hypha vacuoles. Mannitol, peanut meal, beef extract, metal ions (Mn2+, Ca2+, Fe2+, and Mg2+), and amino acids (Arg and Lys) could promote the production of PCN by NF011, moreover, the optimal pH and temperature was 6.0 and 20 ?C. The PCN produced by NF011 under the optimized culture conditions reached 436.55 +/- 11.06 mg/ L, 4.90-fold higher than that observed under the basic culture conditions. Finally, infection experiment results showed that NF011 can effectively prevent Fg spores from infecting wheat spikes and wheat grains and suppress the production of deoxynivalenol (DON). Therefore, the salt-tolerant PCN-producing NF011 has the potential to control wheat fungal disease.