Expression levels and significance of miR-184 and miR-126 in burned rats.

摘要

Expression levels and significance of miR-184 and miR-126 in burned rats were investigated. A total of 30 healthy rats were selected to construct a burn rat model, and another 10 healthy rats as the control group. The modeled rats were divided into groups I, II and III according to burn area, 10 for each group. The reverse transcription-quantitative polymerase chain reaction (RT-q.PCR) was used to detect the expression of miR-184 and miR-126 in the serum of three groups of burned rats, and ELISA was employed to detect the expression levels in peripheral blood and the correlation. There was no difference in the expression levels of miR-184 and miR-126 among the four groups of rats before modeling (P>0.05). Those of miR-184 and miR-126 at each time point were lower than those at the previous one in groups I, II and III (P<0.05). There was no significant change in the expression levels of miR-184 and miR-126 in the control group (P>0.05). At the same time point, those of miR-184 and miR-126 decreased with the increase of burn degree, and the difference was statistically significant between every two groups (P<0.05). The results of Pearson's correlation analysis revealed that the expression level of miR-184 was positively correlated with that of miR-126 (r=0.832, P=0.002). miR-184 and miR-126 were positively correlated with burn degree (r=0.901, P=0.001, r=0.775, P=0.001) and time after burn (r=0.732, P=0.004, r=0.753, P=0.002). The expression levels of miR-184 and miR-126 decrease in burned rats. The changes of their levels may be used as a reference standard for clinical efficacy evaluation to evaluate burn degree, preventing burn wounds from deepening.