Validation of analytical method for determination of querctin concentration in cane sugar and cane juice

摘要

A rapid and sensitive and reliable method was developed for the determination of querctin concentration at different pH of cane sugar and cane juice. The validation of analytical method for the determination of querctin concentration was done by HPLC. The method consisted of extracting with methanol from cane sugar. The extracts were subjected onto column filled with florisil column chromatography. The extraction of querctin was from cane juice with ethyl acetate. Quantification is performed by reversed phase HPLC with UV detector.