-Rapid communication-Complete Cytochrome b Gene Information in the Silky Fowl (Gallus gallus) (Black feather line)

摘要

In accordance with chicken mtDNA sequences, three pairs of primers were designed for the cytochrome b (Cyt b) gene. Three mtDNA fragments were amplified via PCR from silky fowl total genomic DNA in blood. The PCR products were used for detecting the DNA sequences by the direct-sequencing method. The silky fowl cytochrome b gene consisted of 1143 bp nucleotides, encoded for a 380 amino acid protein, and showed 99.4% and 86.8% homology with White Leghorn and Japanese quail, respectively. The homology of the amino acid sequence of the Cyt b gene of silky fowl and White Leghorn birds was 100%, with a corresponding 93.1% between silky fowl and quail. The sequences reported in this paper have been deposited in the GenBank database (accession no. AF 102874).