Metabolic activity of anchorage-dependent animal cells at liquid/liquid interface in long-term culture

摘要

The metabolic activity (glucose consumption and lactate production) of L-929 cells in a liquid/liquid interface system (culture medium/fluorocarbon) has been investigated in long-term culture. The specific glucose consumption rate and specific lactate production rate at the interface were found to be higher than those at a polystyrene surface.This result can be related to the fact that the cell adhesion at theinterface is weakerthan that at a polystyrene surface. It was suggested that cell culture at the interface could significant in raising yields from animal cell bioreactors.