The Culture Medium Conditioned by Adipose Tissue Is Able to Regulate the lncRNA HOTAIR, HOXC11, HOXC12, Osx and SATB2 Gene Expression in MSCs during Osteoblasts' Differentiation

摘要

The osteogenesis is a complex process that involves an accurate control of bone development, growth as well as remodeling during postnatal life. In particular, osteoblasts arise from mesenchymal stem cells through a process regulated in several steps that leads to the osteogenic differentiation. Class I homeobox genes (HOX in human and Hox in mice), are 39 transcription factors, mainly involved in the regulation of embryonic development. It has been shown that HOXA10 was able to activate several osteoblast related genes like Osx. The HOXA10 gene was considered a key factor able to control osteogenesis. In the last decade, several non-coding RNAs (ncRNAs), have been identified like crucial factors during osteogenesis. HOTAIR is an lncRNA related to Osteosarcoma and able to induce the Epithelial Mesenchymal Transition. The aim of this paper was understood the role of HOTAIR and HOXC locus in the control of Osteoblasts development in MSCs treated with adipose tissue obtained with Lipogems method.