Free amino acids, biogenic amines, and ammonium profiling in tobacco from different geographical origins using microwave‐assisted extraction followed by ultra high performance liquid chromatography

Cai Kai; Xiang Zhangmin; Li Hongqin; Zhao Huina; Lin Yechun; Pan Wenjie; Lei Bo

摘要

Abstract > This work describes a rapid, stable, and accurate method for determining the free amino acids, biogenic amines, and ammonium in tobacco. The target analytes were extracted with microwave‐assisted extraction and then derivatized with diethyl ethoxymethylenemalonate, followed by ultra high performance liquid chromatography analysis. The experimental design used to optimize the microwave‐assisted extraction conditions showed that the optimal extraction time was 10?min with a temperature of 60°C. The stability of aminoenone derivatives was improved by keeping the pH near 9.0, and there was no obvious degradation during the 80°C heating and room temperature storage. Under optimal conditions, this method showed good linearity ( R 2 0.999) and sensitivity (limits of detection 0.010–0.081 μg/mL). The extraction recoveries were between 88.4 and 106.5%, while the repeatability and reproducibility ranged from 0.48 to 5.12% and from 1.56 to 6.52%, respectively. The newly developed method was employed to analyze the tobacco from different geographical origins. Principal component analysis showed that four geographical origins of tobacco could be clearly distinguished and that each had their characteristic components. The proposed method also showed great potential for further investigations on nitrogen metabolism in plants. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> >本工作描述了一种快速，稳定，准确的方法，用于确定自由氨基酸，生物生成氨基酸胺和烟草中的铵。用微波辅助萃取萃取靶分析物，然后用二乙氧基甲基丙烯酸乙烯丙酯衍生化，然后用超高效液相色谱分析分析。用于优化微波辅助提取条件的实验设计表明，最佳提取时间为10°C，温度为60℃。通过将pH保持靠近9.0，改善了氨基酮衍生物的稳定性，并且在80℃加热和室温储存期间没有明显的降解。在最佳条件下，该方法显示出良好的线性度（ R <sop> 2 </ sup>＆ 0.999）和灵敏度（检测限值0.010-0.081μg/ ml）。提取回收率在88.4和106.5％之间，而可重复性和再现性分别为0.48至5.12％，分别为1.56至6.52％。采用新开发的方法来分析来自不同地理起源的烟草。主成分分析表明，烟草的四个地理起源可以清楚地区分，每个地理起源都有它们的特征组分。该方法还表明，进一步研究植物中氮代谢的进一步调查潜力巨大。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-35800/'>《Journal of separation science.》</a> <b style="margin: 0 2px;">|</b><span>2017年第23期</span><b style="margin: 0 2px;">|</b><span>共12页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Cai Kai&option=202" target="_blank" rel="nofollow">Cai Kai;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Xiang Zhangmin&option=202" target="_blank" rel="nofollow">Xiang Zhangmin;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li Hongqin&option=202" target="_blank" rel="nofollow">Li Hongqin;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhao Huina&option=202" target="_blank" rel="nofollow">Zhao Huina;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lin Yechun&option=202" target="_blank" rel="nofollow">Lin Yechun;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Pan Wenjie&option=202" target="_blank" rel="nofollow">Pan Wenjie;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lei Bo&option=202" target="_blank" rel="nofollow">Lei Bo;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Guizhou Academy of Tobacco ScienceGuiyang P. R. China;</p> <p>Guizhou Academy of Tobacco ScienceGuiyang P. R. China;</p> <p>Yibin County Testing and Inspection Center for Agricultural Products QualityYibin P. R. China;</p> <p>Guizhou Academy of Tobacco ScienceGuiyang P. R. China;</p> <p>Guizhou Academy of Tobacco ScienceGuiyang P. R. China;</p> <p>Guizhou Academy of Tobacco ScienceGuiyang P. R. China;</p> <p>Guizhou Academy of Tobacco ScienceGuiyang P. R. China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/159.html" title="化学">化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=amino acids&option=203" rel="nofollow">amino acids;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=biogenic amines&option=203" rel="nofollow">biogenic amines;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=geographical origin&option=203" rel="nofollow">geographical origin;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=microwave‐assisted extraction&option=203" rel="nofollow">microwave‐assisted extraction;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=tobacco&option=203" rel="nofollow">tobacco;</a> </p> <div class="translation"> 机译：氨基酸;生物胺;地理来源;微波辅助提取;烟草; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025845813.html">Free amino acids, biogenic amines, and ammonium profiling in tobacco from different geographical 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chromatographic method</a> <b>[P]</b> . <span> 外国专利：  US2007281361A1 </span> <span> . 2007-12-06</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：柱前衍生试剂-1-萘基异氰酸酯与高效液相色谱法测定人血浆游离氨基酸 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130437612466.html">method for the simultaneous determination of purine derivatives and pirimidine, amino acid n - acetylated and dicarbossilici acids in a biological sample by high-performance liquid chromatography (hplc) and high-performance on kit.</a> <b>[P]</b> . <span> 外国专利：  ITRM20050085A1 </span> <span> . 2006-09-01</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：高效液相色谱法（hplc）和高性能试剂盒同时测定生物样品中嘌呤衍生物和嘧啶，氨基酸正乙酰化和二碳碳硅酸的方法 </span> </p> </li> </ul> </div> </div> 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