Real‐time metabolite monitoring of glucose‐fed <fc xmlns='http://www.wiley.com/namespaces/wiley'> <fi>Clostridium acetobutylicum</fi> </fc> <fi xmlns='http://www.wiley.com/namespaces/wiley'>Clostridium acetobutylicum</fi>Clostridium acetobutylicum fermentations using Raman assisted metabolomics

Zu Theresah N.K.; Liu Sanchao; Gerlach Elliot S.; Germane Katherine L.; Servinsky Matthew D.; Mackie David M.; Sund Christian J.

摘要

Abstract > Data obtained from in situ Raman spectroscopy probes and high‐performance liquid chromatography (HPLC) analysis were applied together with chemometrics to build partial least squares models of metabolite concentrations for the industrially relevant organism <fi>Clostridium acetobutylicum</fi> . Models were built for predominant products (acetic acid, butyric acid, and butanol) of <fi>C.?acetobutylicum</fi> cultures grown on glucose as a substrate. The partial least squares models were then applied to a 3‐day <fi>C.?acetobutylicum</fi> culture for real‐time, quantitative metabolite analysis. The predicted outcomes of new fermentation cultures were validated by analyzing HPLC data from corresponding experiments from these new fermentation cultures. Model predictions showed good correlation with measured data (goodness of fit [R2Y] values of 0.99, and goodness of prediction [Q2Y] values of 0.98 from agitated cultures. Predictive models based upon Raman spectral data are promising tools for characterization of synthetic organisms, guiding process control, and facilitating optimization of culture conditions. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> >数据从原位拉曼光谱探针和高性能液相色谱（HPLC）分析与化学计量学相同，以构建工业相关的生物体的代谢物浓度的偏最小二乘模型<FI>酸梭菌乙酰丁基 - / FI>。为在葡萄糖中生长的主要产品（乙酸，丁酸，丁醇）的主要产品（乙酸，丁酸和丁醇）构建为<fi> c.?乙酰丁酯的<fi> c.?乙酰丁酯</ fi>培养物。然后将部分最小二乘模型应用于3天<FI> C.?乙酰丁基蛋白</ Fi>实时，定量代谢物分析。通过从这些新发酵培养物的相应实验中分析HPLC数据来验证新发酵培养物的预测结果。模型预测显示出与测量数据的良好相关性（适合拟合[R2Y]值0.99的值，以及从搅拌培养的预测值0.98的高度。基于拉曼光谱数据的预测模型是用于表征合成生物的有希望的工具，引导过程控制，促进培养条件的优化。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-35645/'>《Journal of Raman Spectroscopy: An International Journal for Original Work in All Aspects of Raman Spectroscopy, Including Higher Order Processes, and Also Brillouin- and Rayleigh Scattering》</a> <b style="margin: 0 2px;">|</b><span>2017年第12期</span><b style="margin: 0 2px;">|</b><span>共11页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zu Theresah N.K.&option=202" target="_blank" rel="nofollow">Zu Theresah N.K.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liu Sanchao&option=202" target="_blank" rel="nofollow">Liu Sanchao;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gerlach Elliot S.&option=202" target="_blank" rel="nofollow">Gerlach Elliot S.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Germane Katherine L.&option=202" target="_blank" rel="nofollow">Germane Katherine L.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Servinsky Matthew D.&option=202" target="_blank" rel="nofollow">Servinsky Matthew D.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mackie David M.&option=202" target="_blank" rel="nofollow">Mackie David M.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sund Christian J.&option=202" target="_blank" rel="nofollow">Sund Christian J.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>U.S. Army Research Laboratory Sensors and Electron Devices DirectorateBiotechnology Branch Bio‐Systems and Bio‐Integration TeamAdelphi MD USA;</p> <p>U.S. Army Research Laboratory Sensors and Electron Devices DirectorateBiotechnology Branch Bio‐Systems and Bio‐Integration TeamAdelphi MD USA;</p> <p>U.S. Army Research Laboratory Sensors and Electron Devices DirectorateBiotechnology Branch Bio‐Systems and Bio‐Integration TeamAdelphi MD USA;</p> <p>U.S. Army Research Laboratory Sensors and Electron Devices DirectorateBiotechnology Branch Bio‐Systems and Bio‐Integration TeamAdelphi MD USA;</p> <p>U.S. Army Research Laboratory Sensors and Electron Devices DirectorateBiotechnology Branch Bio‐Systems and Bio‐Integration TeamAdelphi MD USA;</p> <p>U.S. Army Research Laboratory Sensors and Electron Devices DirectorateBiotechnology Branch Bio‐Systems and Bio‐Integration TeamAdelphi MD USA;</p> <p>U.S. Army Research Laboratory Sensors and Electron Devices DirectorateBiotechnology Branch Bio‐Systems and Bio‐Integration TeamAdelphi MD USA;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/4724.html" title="光谱学">光谱学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=chemometrics&option=203" rel="nofollow">chemometrics;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Clostridium&option=203" rel="nofollow">Clostridium;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=fermentation&option=203" rel="nofollow">fermentation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=MVDA&option=203" rel="nofollow">MVDA;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=predictive modeling&option=203" rel="nofollow">predictive modeling;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=R aman spectroscopy&option=203" rel="nofollow">R aman spectroscopy;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=real‐time monitoring&option=203" rel="nofollow">real‐time monitoring;</a> </p> <div class="translation"> 机译：化学计量学;Clostridium;发酵;MVDA;预测建模;R aman光谱;实时监测; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li 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Real‐time metabolite monitoring of glucose‐fed Clostridium acetobutylicum Clostridium acetobutylicumClostridium acetobutylicum fermentations using Raman assisted metabolomics

摘要