Prevalence of Mycoplasmosis and Antibiotic Susceptibility of Mycoplasma gallisepticum in Commercial Chicken Flocks of Rawalpindi Division, Pakistan

摘要

Involvement of Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) in respiratory disease in commercial chicken showing respiratory signs was investigated using polymerase chain reaction (PCR). Out of the 124 suspected cases, 50 (40.32%) were found to be positive for MG and 17 (13.70%) were positive for MS. The MG isolates were recovered from birds and subjected to antibiotic sensitivity testing by the broth microdilution method. Tilmicosin with MIC 90 and MIC50 values of 3.12 and 6.25 mu g/ml were found to be the most effective drug, followed by tylosin, erythromycin and enrofloxacin, all of which gave MIC% and MIC50 values of 6.25 and 12.5 mu g/ml respectively. Oxytetracycline and chlortetracycline were found to be the least effective drugs and the MIC% value for both these antibiotics was 100.0. The MIC values for various antibiotics recorded in this study were generally higher compared to those found in previous investigations. (C) 2018 PVJ. All rights reserved