Comparative sensitivity and inhibitor tolerance of GlobalFiler (R) PCR Amplification and Investigator (R) 24plex QS kits for challenging samples

摘要

In cases such as mass disasters or missing persons, human remains are challenging to identify as they may be fragmented, burnt, been buried, decomposed, and/or contain inhibitory substances. This study compares the performance of a relatively new STR kit in the US market (Investigator (R) 24plex QS kit; Qiagen) with the GlobalFiler (R) PCR Amplification kit (Thermo Fisher Scientific) when genotyping highly inhibited and low level DNA samples.