Differential toxicity on monocytes and monocyte-derived dendritic Cells:a new tool to differentiate allergens from irritants?

摘要

Phenotypic activation of monocyte-derived dendritic cells has been proposed as an in vitro alternative assay to discriminate potential sensitizers from irritants,but the sensitivity of the assay remains controversial.In this study,we first determined the dynamic range of expression of activation/maturation markers on human monocyte-derived dendritic cells cultured in the presence or absence of transforming growth factor ss(TGF beta)?On day three of culture,most monocytes had already differentiated into dendritic cells that expressed low levels of costimulatory molecules especially in the presence TGF beta-treatment of 3-day-old TGF beta-treated monoevte-derived dendritic cells with several chemicals at sub-toxic concentrations induced significant phenotypic changes for all the strong and mild sensitizers tested,whereas the irritant sodium lauryl sulfate had no effect.