ASSESSMENT OF CANCER TARGETING POTENTIAL OF DOXORUBICIN CONJUGATED WITH SURFACE FUNCTIONALIZED MULTI-WALLED CARBON NANOTUBES

摘要

Our main aim in the present investigation was to assess the cancer targeting potential of doxorubicin conjugated with folic acid (FA), ethylene di-amine (EDA) and surface F- MWCNTs (FA-EDA-MWCNTs-DOX) conjugate employing on MCF-7 (breast cancer cell line) for efficient tumor targeting. We developed a highly effective novel targeted drug delivery based on DOX-conjugated with the surface of F-MWCNTs by using nucleophilic substitution reaction mechanism and evaluated in facile strategy for cancer treatment. The in vitro drug release study shows that the percentage of drug release under an acidic condition pH-5.4 is higher than that under normal physiological conditions. The FA-EDA-MWCNTs-DOX nano-conjugate affords higher efficacy in tumor growth suppression due to its stealth nature and most preferentially taken up by cultured MCF-7 cells through receptor-mediated endocytosis mechanism. Fourier Transform Infrared Spectroscopy (FTIR), UV-Visible Spectroscopy, Nuclear Magnetic Resonance Spectroscopy (NMR) and Scanning Electron Microscopy (SEM) measurements clearly confirmed the functionalization & conjugation steps. The results concluded that developed water-soluble nano-conjugate might emerge as "safe and effective" nano-medicine in cancer treatment by minimizing the side effects with generally regarded as a safe prominence.