MICROBIAL BIOSENSORS FOR ENVIRONMENTAL MONITORING

摘要

Microbial biosensors are analytical devices capable of sensing substances in the environment due to the specific biological reaction of the microorganism or its parts. Construction of a microbial biosensor requires knowledge of microbial response to the specific analyte. Linking this response with the quantitative data, using a transducer, is the crucial step in the construction of a biosensor. Regarding the transducer type, biosensors are divided into electrochemical, optical biosensors and microbial fuel cells. The use of the proper configuration depends on the selection of the biosensing element. With the use of transgenic E. coli strains, bioluminescence or fluorescence based biosensors were developed. Microbial fuel cells enable the use of theheterogeneous microbial populations, isolated from wastewater. Different microorganisms are used for different pollutants - pesticides, heavy metals, phenolic compounds, organic waste, etc. Biosensing enables measurement of their concentration and theirtoxic or genotoxic effects on the microbes. Increasing environmental awareness has contributed to the increase of interest for biomonitoring. Although technologies, such as bioinformatics and genetic engineering, allow us to design complex and efficientmicrobial biosensors for environmental pollutants, the transfer of the laboratory work to the field still remains a problem to solve.