The conjunction of a new ultrasonic-assisted dispersive solid-phase extraction method with HPLC-DAD for the trace determination of diazinon in biological and water media

摘要

Herein, a new ultrasonic-assisted dispersive solid-phase extraction method using a TiO2/magnetic graphene-based sorbent in conjunction with the HPLC-diode array detection (HPLC-DAD) was successfully developed for the trace detection of diazinon. Initially, the TiO2/magnetic graphene-based sorbent was successfully synthesized and subsequently characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), energy-dispersive X-ray spectroscopy (EDS) and elemental mapping techniques. Then, the sorbent was used for the sorption and extraction of diazinon mainly through - stacking hydrophobic interactions. Under the optimized conditions, the calibration curves were linear over the concentration range of 0.8-800 g L-1 for urine, plasma and milk samples and 0.2-200 g L-1 for water samples. The method detection limits were determined to be in the range of 0.05-0.3 g L-1 based on a signal-to-noise ratio of 3 (S/N = 3). To test the extraction efficiency, the method was applied to various fortified real samples. The average recoveries obtained from the fortified samples varied between 92-104% with relative standard deviations of 6.1-10.2%. Finally, the method was determined to be effective for the analysis of diazinon in environmental and biological samples.