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Ethanol Production from Ulva fasciata

机译:阔叶锦葵的乙醇生产

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摘要

The theoretical potential yield of Ulva fasciata as a biomass feedstock for fermentative ethanol was found to be about 310 L per tonne, dry weight. U. fasciata has numerous characteristics that render it a suitable mariculture energy crop. Specifically, it forms large complex structures that grow quickly, with high (14%) dry to wet weight percentages, holocellulose content for the dry mass of 51%, carbohydrate content of 5%, and relatively low (5%) lignin content. Enzymatic saccharification with a commercial cellulase (Accelerase) from Genencor was investigated: After a 12 hr digestion, 2.5% of the potential glucose was recovered from the cellulose fraction. The hydrolysate was supplemented with a modified YM medium and used directly for batch fermentation. A 12 hr incubation resulted in complete utilization of the glucose and production of ethanol. In this preliminary investigation, the ethanol yield corresponded to approximately 126 L per tonne (dry weight) of macroalga, or ~43% of the theoretical alcohol yield with respect to only the cellulose and carbohydrate contents. Theoretical yields are higher when the hemicellulose fraction is considered. While sugar recovery needs further optimization, the data suggest that additional work is warranted.
机译:发现Ulva fasciata作为发酵乙醇的生物质原料的理论潜在产量为每吨干重约310L。 fasciata具有许多特征,使其成为适宜的海水养殖能源作物。具体而言,它形成了快速增长的大型复杂结构,干重比湿重百分比高(14%),干质量的全纤维素含量为51%,碳水化合物含量为5%,木质素含量相对较低(5%)。用来自Genencor的商业纤维素酶(Accelerase)酶促糖化进行了研究:消化12小时后,从纤维素级分中回收了2.5%的潜在葡萄糖。在水解产物中添加改良的YM培养基,并直接用于批量发酵。孵育12小时会导致葡萄糖的完全利用和乙醇的产生。在这项初步研究中,乙醇产量相当于每吨大藻(干重)约126 L,或仅相对于纤维素和碳水化合物含量而言约为理论醇产量的43%。当考虑半纤维素部分时,理论产率较高。尽管糖回收需要进一步优化,但数据表明需要做更多的工作。

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