Peroxides in mineral oil used for in vitro fertilization: defining limits of standard quality control assays

摘要

Purpose To determine the relative sensitivities of the 1 and 2-cell mouse embryo assays (MEA) and the human sperm motility assay (HSMA) for peroxides in mineral oil. The effect of peroxide on blastocyst cell number and apoptosis was also studied.Methods One and two-cell MEA and HSMA were performed using mineral oil containing cumene hydroperoxide (CH).