Effect of eyestalk ablation on maturation, larval performance, and biochemistry of white Pacific shrimp, Penaeus vannamei, broodstock

摘要

During a production period in a commercial Mexican hatchery, several traits of females, ablated by cutting one eyestalk at its base (n = 37) and unablated controls (n = 60), were analysed. These analyses included reproductive performance, biochemical characteristics of eggs and nauplii, and larvae survival. In addition, possible long-term physiological alterations of spawners were evaluated using histological and biochemical approaches. The number of spawns a female had or the maturation stage of spawners were also considered as additional factors influencing the variables recorded. Ablated females had more frequent spawns (P<0.01) and a higher gonadosomatic index (P<0.05) but a lower weight gain (P<0.05) than unablated controls. Ablated females had lower levels of haemocyanin in haemolymph (P<0.01) and of glucose in hepatopancreas (P<0.05) but higher levels of total carotenoids in hepatopancreas (P<0.05) than controls. The eggs of ablated females had higher levels of acylglycerides (P<0.05) thaneggs of control females. There were some differences in the biochemical levels in tissues and eggs as functions of the number of spawns between ablated and unablated females. No differences in the biochemical composition of nauplii or in the larval survival during culture to postlarvae stage 1 were found between ablated and unablated females.