A Rapid Spectrophotometric Method to Resolve a Binary Mixture of Food Colorants (Riboflavine and Sunset Yellow)

摘要

A very simple and rapid Spectrophotometric method using measurements at zero-crossing wavelength is described for resolving binary mixtures of riboflavine (E-101) and sunset yellow (E-110) in a powder drink.The assay procedure for E-110 and E-101 involves the extraction of the colorants from the powder drink with pH 4.5 acetate buffer,filtration,appropriate dilution,and measurement of the first derivative absorbance values (denoted as ~1D).Calibration graphs are linear up to 25.0 mug.mL~(-1) for E-101 and 40.0 mug.mL~(-1) for E-110.The sensitivity and reproducibility of the method were determined by using the synthetic mixtures in different ratios prepared by us,and the proposed method was applied to a commercially available powder drink.The relative standard deviations obtained are 1.39% and 1.15% and the average percentage recoveries are 101.0% and 102.8% for E-101 and E-110,respectively.