PSEUDORABIES VIRUS EPO IS FUNCTIONALLY HOMOLOGOUS TO VARICELLA-ZOSTER VIRUS ORF61 PROTEIN AND HERPES SIMPLEX VIRUS TYPE 1 ICPO

摘要

Pseudorabies virus (PRV) early protein 0 (EP0) is the homolog of varicella-zoster virus (VZV) open reading frame 61 (ORF61) protein and herpes simplex virus type 1 (HSV-1) ICP0. A PRV EP0 deletion mutant grows poorly in cell culture, suggesting that EP0 plays a critical role in the viral replicative cycle, In this study, we have shown that the growth defect of an EP0 deletion mutant was complemented in Vero cells expressing VZV ORF61 protein or HSV-1 ICP0. In transient expression assays PRV EP0, like VZV ORF61 protein and HSV-1 ICP0, transactivates a variety of promoters from PRV, VZV, HSV, and unrelated viruses. These data indicate that PRV EP0 is functionally homologous to VZV ORF61 protein and HSV-1 ICP0. (C) 1995 Academic Press, Inc.