Structural analysis of the core region of the lipopolysaccharides from eight serotypes of Klebsiella pneumoniae

摘要

The core regions of the lipopolysaccharides (LPS) from Klebsiella pneumoniae serotypes O1,O2a,O2z,c,O3,O4,O5,O8,and O12 were analysed using NMR spectroscopy,ESI-MS spectroscopy,and chemical methods.All the LPSs had similar core structures,as shown below,differing only in thenumber and position of #beta#-D-galacturonic acid substitutents:where P is H or #alpha#-Hep,J.K, is H or #beta#-GalA.LPS from all serotypes contained varing proportions of structures having additional or missing phosphate substitutents.The core from serotype O1 contained a minor amount of a previously described variant with #alpha#-DD-Hep-(1->2)-#alpha#-DD-Hep-(1->6)-#alpha#-G1cN-(1->replacing the #alpha#-Hep(1->4)-#alpha#-Kdo-(2->6)-#alpha#-G1cN-(1-component).