A new fluorescent assay for sialyltransferase

摘要

A new fluorescent assay for the sialyltransferase reaction was established. After incubationof the sialyltransferase reaction, the sialyloligosaccharide obtained was treated by acid hydrolysis, and then the NeuAc that was released was labeled with 1,2-diamino-4,5-methylenedioxibenzene. The fluorescent-labeled NeuAc could be estimated by HPLC (excitation: 373 nm; emission; 448 nm) and a Lineweaver-Burk plot could be plotted with the data from this analysis.