A Simple Method for Measuring Long-Range ~1H-~(13)C Coupling Constants in Organic Molecules

摘要

This study presents a simple method for measuring long-range heteronuclear coupling constants between protons and proton-bearing carbons. The approach involves recording two conventional 1D-TOCSY experiments in which the offset of the selective proton pulse is set on the low- and high-frequency ~(13)C satellites of an isolated proton signal, Hi. Long-range heteronuclear coupling values between the ~(13)Ci bonded to Hi and the protons Hj,k...n that belong to the same spin system were easily determined from the relative displacement of the relayed Hj,k...n signals in the satellite-selective TOCSY spectra. The sense of the displacement indicated the sign of the coupling constants.