Cysteine synthase overexpression in tobacco confers tolerance tosulfur-containing environmental pollutants

摘要

Cysteine (Cys) synthase [O-acetyl-L-Ser(thiol)-lyase, EC 4.2.99.8; CSase] is responsible for the final step in biosynthesis of Cys. Transgenic tobacco (Nicotiana tabacum; F-1) plants with enhanced CSase activities in the cytosol and in the chloroplasts were generated by cross-fertilization of two transformants expressing cytosolic CSase or chloroplastic CSase. The F-1 transgenic plants were highly tolerant to toxic sulfur dioxide and sulfite. Upon fumigation with 0.1 muL L-1 sulfur dioxide, the Cys and glutathione contents in leaves of F-1 plants were increased significantly, but not in leaves of non-transformed control plants. Furthermore, the leaves of F-1 plants exhibited the increased resistance to paraquat, a herbicide generating active oxygen species.