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Molecular Biotechnology

Molecular Biotechnology

中文名称：分子生物技术

ISSN：1559-0305
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1.Design and Implementation of High Throughput Screening Assays

机译：高通量筛选分析的设计与实现
- 作者：Ricardo Macarrón;Robert P. Hertzberg
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- Bioassay;
- Cell culture;
- Drug screening;
- Human;
- Methodology;
- Reproducibility;
2.E. coli-Based Cell-Free Expression, Purification and Characterization of the Membrane-Bound Ligand-Binding CHASE-TM Domain of the Cytokinin Receptor CRE1/AHK4 of Arabidopsis thaliana

机译：拟南芥细胞分裂素受体CRE1 / AHK4的膜结合配体结合CHASE-TM域的基于大肠杆菌的无细胞表达，纯化和表征
- 作者：Klaas Wulfetange;Wolfram Saenger;Thomas Schmülling;Alexander Heyl
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- Cell-free expression;
- CHASE domain;
- Cytokinin;
- Hormone receptor;
- Membrane protein;
3.IAP Display: A Simple Method to Identify Mouse Strain Specific IAP Insertions

机译：IAP显示：一种简单的方法来识别特定于鼠标应变的IAP插入
- 作者：Akshay Ray;Raheleh Rahbari;Richard M. Badge
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- IAP element;
- Retrotransposon;
- Inbred mouse strain;
- Genetic identification;
- Display PCR;
4.Selection of Reference Genes for qRT-PCR in High Fat Diet-Induced Hepatic Steatosis Mice Model

机译：高脂饮食诱导的肝脂肪变性小鼠模型中qRT-PCR参考基因的选择
- 作者：Lingyan Xu;Xinran Ma;Bin Cui;Xiaoying Li;Guang Ning;Shu Wang
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- Hepatic steatosis;
- Gene expression;
- Quantitative real-time PCR;
- Housekeeping genes;
- Normalization;
5.A Flexible Approach to Studying Post-Transcriptional Gene Regulation in Stably Transfected Mammalian Cells

机译：在稳定转染的哺乳动物细胞中研究转录后基因调控的灵活方法
- 作者：Ralph C. Nichols;John Botson;Xiao Wei Wang;B. JoNell Hamilton;Jane E. Collins;Victoria Uribe;Seth A. Brooks;Moe Zan;William F. C. Rigby
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- ARE;
- TNF;
- mRNA turnover;
- Translation;
- 3′-UTR;
6.Ribosome Display Selection of a Murine IgG1 Fab Binding Affibody Molecule Allowing Species Selective Recovery Of Monoclonal Antibodies

机译：鼠IgG1 Fab结合亲和分子的核糖体展示选择，允许物种选择性回收单克隆抗体。
- 作者：Sebastian Grimm;Feifan Yu;Per-Åke Nygren
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- Mouse IgG1;
- Affibody;
- Selection;
- Fab fragment;
- Combinatorial protein engineering;
- Ribosome display;
7.Identification of Pathogenicity-Related Genes in the Vascular Wilt Fungus Verticillium dahliae by Agrobacterium tumefaciens-Mediated T-DNA Insertional Mutagenesis

机译：农杆菌介导的T-DNA插入诱变技术鉴定大黄萎病菌中黄萎病病原相关基因
- 作者：K. Maruthachalam;S. J. Klosterman;S. Kang;R. J. Hayes;K. V. Subbarao
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- Verticillium dahliae;
- Lactuca sativa;
- ATMT;
- Pathogenicity related genes;
8.Agrobacterium-Mediated Silencing of Caffeine Synthesis through Root Transformation in Camellia sinensis L.

机译：农杆菌介导的茶树咖啡因合成中的咖啡因合成沉默。
- 作者：Prashant Mohanpuria;Vinay Kumar;Paramvir Singh Ahuja;Sudesh Kumar Yadav
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- Agrobacterium;
- Caffeine synthase;
- Caffeine silencing;
- Camellia sinensis;
- Root transformation;
9.Refolding by High Pressure of a Toxin Containing Seven Disulfide Bonds: Bothropstoxin-1 from Bothrops jararacussu

机译：通过高压重折叠包含七个二硫键的毒素：来自Bothrops jararacussu的Bothropstoxin-1
- 作者：Keli N. Balduino;Patrick J. Spencer;Natalia V. Malavasi;Rosa M. Chura-Chambi;Laura S. Lemke;Ligia Morganti
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- High hydrostatic pressure;
- Refolding;
- Myotoxin;
- K49 phospholipase;
- Aggregation;
10.Enhanced Production of Human Recombinant Proteins from CHO cells Grown to High Densities in Macroporous Microcarriers

机译：大孔微载体中生长到高密度的CHO细胞增强人重组蛋白的生产
- 作者：T. Tharmalingam;K. Sunley;M. Spearman;M. Butler
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- CHO;
- Microcarrier;
- Cytopore;
- Tissue plasminogen activator;
- Beta-interferon;
11.Identification of MicroRNA Target Genes in Vivo

机译：体内MicroRNA靶基因的鉴定
- 作者：Wei Zheng;Hua-Wei Zou;Yong-Gang Tan;Wei-Song Cai
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- MicroRNA;
- Biotin;
- Target isolation;
- hsa-miR155;
12.Evidence of the Involvement of Asparagine Deamidation in the Formation of Cyclodextrin Glycosyltransferase Isoforms in Paenibacillus sp. RB01

机译：天冬酰胺脱酰胺参与Paenibacillus sp。形成环糊精糖基转移酶同工型的证据。 RB01
- 作者：Wanchai Yenpetch;Kanoktip Packdibamrung;Wolfgang Zimmermann;Piamsook Pongsawasdi
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- Paenibacillus sp. RB01;
- Cyclodextrin glycosyltransferase;
- Deamidation;
- Isoform formation;
- Isoaspartate;
13.Clones Identification and Genetic Characterization of Garnacha Grapevine by Means of Different PCR-Derived Marker Systems

机译：利用不同的PCR衍生标记体系鉴定Garnacha葡萄的克隆和遗传特性
- 作者：Stefano Meneghetti;Angelo Costacurta;Enrica Frare;Graziana Da Rold;Daniele Migliaro;Giacomo Morreale;Manna Crespan;Vicente Sotés;Antonio Calò
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- Cannonau;
- Grenache;
- AFLP;
- M-AFLP;
- SAMPL;
- Intravarietal variability;
14.Expression of a Cholera Toxin B Subunit-Neutralizing Epitope of the Porcine Epidemic Diarrhea Virus Fusion Gene in Transgenic Lettuce (Lactuca sativa L.)

机译：猪流行性腹泻病毒融合基因的霍乱毒素B亚基中和表位在转基因生菜中的表达
- 作者：Nguyen-Xuan Huy;Moon-Sik Yang;Tae-Geum Kim
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- Edible vaccine;
- Transgenic lettuce;
- Cholera toxin B subunit;
- PEDV;
- COE;
15.One-Step Detection of Aflatoxin-B1 Using scFv-Alkaline Phosphatase-Fusion Selected from Human Phage Display Antibody Library

机译：从人噬菌体展示抗体库中选择的scFv-碱性磷酸酶融合蛋白一步检测黄曲霉毒素B1
- 作者：Kuntalee Rangnoi;Nanthnit Jaruseranee;Richard O’Kennedy;Potjamas Pansri;Montarop Yamabhai
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- Phage display;
- scFv;
- Antibody engineering;
- Alkaline phosphatase;
- ELISA;
- Aflatoxin;
- Human;
- Hapten;
- Competitive;
- Recombinant antibody;
16.Comparative Analysis of Transduced Primary Human Dendritic Cells Generated by the Use of Three Different Lentiviral Vector Systems

机译：使用三种不同的慢病毒载体系统产生的转导人类树突状细胞的比较分析
- 作者：Elena Grabski;Zoe Waibler;Silke Schüle;Björn-Philipp Kloke;Linda Y. Sender;Sylvia Panitz;Klaus Cichutek;Matthias Schweizer;Ulrich Kalinke
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- Human dendritic cells;
- Transduction efficiency;
- Lentiviral vectors;
- Immune function;
17.pYEMF, a pUC18-Derived XcmI T-Vector for Efficient Cloning of PCR Products

机译：pYEMF，pUC18衍生的XcmI T载体，可高效克隆PCR产物
- 作者：Jingsong Gu;Chunjiang Ye
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- PCR product;
- Cloning;
- T-vector;
- XcmI;
18.Erratum to: Metabolic Rates, Growth Phase, and mRNA Levels Influence Cell-Specific Antibody Production Levels from In Vitro-Cultured Mammalian Cells at Sub-Physiological Temperatures

机译：勘误如下：在亚生理温度下，代谢率，生长期和mRNA水平影响体外培养的哺乳动物细胞的细胞特异性抗体产生水平。
- 作者：Rosalyn J. Marchant;Mohamed B. Al-Fageeh;Michele F. Underhill;Andrew J. Racher;C. Mark Smales
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
19.One-Step Construction of Lentiviral Reporter Using Red-Mediated Recombination

机译：使用红色介导的重组一步构建慢病毒记者。
- 作者：Juanmin Zha;Xin Chen;Chaojun Li;Minsheng Zhu;Guoxian Ding;Weiqi He
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- Lentiviral vectors;
- Red-mediated system;
- Recombination;
- EGFP;
- Myosin light chain kinase;
20.Musa Genetic Diversity Revealed by SRAP and AFLP

机译：SRAP和AFLP揭示的穆萨遗传多样性
- 作者：Muhammad Youssef;Andrew C. James;Renata Rivera-Madrid;Rodomiro Ortiz;Rosa María Escobedo-GraciaMedrano
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- AFLP;
- Banana;
- Genetic variation;
- Musa;
- Germplasm collection;
- SRAP;
21.A Novel and Simple PCR Walking Method for Rapid Acquisition of Long DNA Sequence Flanking a Known Site in Microbial Genome

机译：一种新颖且简单的PCR步移方法，用于快速获取微生物基因组中已知位点两侧的长DNA序列
- 作者：Peng Luo;Ting Su;Chaoqun Hu;Chunhua Ren
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- Flanking sequence;
- Long PCR;
- Walking;
- Microbial genome;
- Vibrio vulnificus;
- Vibrio alginolyticus;
- Vibrio cholerae;
22.InTRONs in Biotech

机译：生物技术领域的InTRON
- 作者：Natasa Skoko;Marco Baralle;Sergio Tisminetzky;Emanuele Buratti
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- mRNA splicing;
- mRNA stability;
- Introns;
- Exons;
- Gene expression;
23.Reactivation of Transgene Expression by Alleviating CpG Methylation of the Rous sarcoma virus Promoter in Transgenic Quail Cells

机译：通过减轻转基因鹌鹑细胞中劳斯肉瘤病毒启动子的CpG甲基化来激活转基因表达
- 作者：Hyun-Jun Jang;Jin Won Choi;Young Min Kim;Sang Su Shin;Kichoon Lee;Jae Yong Han
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- Transgenic quail;
- RSV promoter;
- Transgene expression;
- CpG methylation;
- 5-aza-2′-deoxycytidine;
24.Characterization of a New α-l-Arabinofuranosidase from Penicillium sp. LYG 0704, and their Application in Lignocelluloses Degradation

机译：青霉属一种新的α-1-阿拉伯呋喃糖苷酶的表征。 LYG 0704及其在木质纤维素降解中的应用
- 作者：Dae-Seok Lee;Seung-Gon Wi;Yoon-Gyo Lee;Eun-Jin Cho;Byung-Yeoup Chung;Hyeun-Jong Bae
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- α-l-Arabinofuranosidase;
- Synergistic effect;
- Xylanase;
- Cellulase;
- Lignocelluloses;
- Enzymatic hydrolysis;
25.Allelic Polymorphism Detected in the Bovine FTO Gene

机译：牛FTO基因中检测到的等位基因多态性
- 作者：Bao Zhang;Ya Zhang;Liangzhi Zhang;Jing Wang;Zhuanjian Li;Hong Chen
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- Cattle;
- FTO gene;
- SNP (single nucleotide polymorphism);
- PCR-SSCP;
26.The Use of the Condensed Single Protein Production System for Isotope-Labeled Outer Membrane Proteins, OmpA and OmpX in E. coli

机译：浓缩单蛋白生产系统用于大肠杆菌中同位素标记的外膜蛋白，OmpA和OmpX的使用
- 作者：S. Thangminlal Vaiphei;Yuefeng Tang;Gaetano T. Montelione;Masayori Inouye
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- NMR;
- OmpA;
- OmpX;
- MazF;
27.Strategies for Optimal Expression of Vaccine Antigens from Taeniid Cestode Parasites in Escherichia coli

机译：大肠杆菌中Ta虫的est虫寄生虫疫苗抗原的最佳表达策略
- 作者：Charles Gauci;David Jenkins;Marshall W. Lightowlers
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- Recombinant;
- Vaccine;
- Antigen expression;
- Parasite;
- Escherichia;
- Taenia;
- Echinococcus;
- Cysticercosis;
28.A New Method for Repeated “Self-Cloning” Promoter Replacement in Saccharomyces cerevisiae

机译：酿酒酵母中重复“自克隆”启动子替换的新方法
- 作者：Olga A. Sofyanovich;Hiroaki Nishiuchi;Kazuo Yamagishi;Kenjiro Maekawa;Vsevolod A. Serebryanyy
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- Promoter replacement;
- Self-cloning;
- Saccharomyces cerevisiae;
- PCR;
29.Characterization and Engineering of a Novel Pyrroloquinoline Quinone Dependent Glucose Dehydrogenase from Sorangium cellulosum So ce56

机译：一种新型的吡咯喹啉醌依赖性葡萄糖脱氢酶的表征与工程
- 作者：Michael Hofer;Kathrin Bönsch;Thomas Greiner-Stöffele;Meike Ballschmiter
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- PQQ;
- Glucose dehydrogenase;
- Sorangium cellulosum;
- Biosensor;
- Substrate specificity;
30.Enhanced Expression of AtNHX1, in Transgenic Groundnut (Arachis hypogaea L.) Improves Salt and Drought Tolerence

机译：AtNHX1在转基因花生中的增强表达提高了耐盐和耐旱性
- 作者：Muhammad Ahsan Asif;Yusuf Zafar;Javaid Iqbal;Muhammad Munir Iqbal;Umer Rashid;Ghulam Muhammad Ali;Anjuman Arif;Farhat Nazir
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- Salt tolerance;
- Arachis hypogaea;
- Drought tolerance;
- Vacuolar Na+/H+ antiporter;
31.Deciphering Enzyme Function Using Peptide Arrays

机译：使用肽阵列解密酶功能
- 作者：Alexandra Thiele;Gabriele I. Stangl;Mike Schutkowski
- 期刊名称：《Molecular Biotechnology》 | 2011年第3期
- Substrate specificity;
- Kinase;
- Protease;
- Phosphatase;
- Peptide microarray;
- SPOT synthesis;
- Kinome profiling;

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