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Molecular Biotechnology

Molecular Biotechnology

中文名称：分子生物技术

ISSN：1559-0305
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1.Comparison of Manual and Automatic (MagNA Pure) Isolation Methods of Total RNA from Adipose Tissue

机译：手动和自动（MagNA Pure）从脂肪组织中提取总RNA的方法的比较
- 作者：Zdenka Lacinova;Marketa Dolinkova;Denisa Haluzikova;Jana Krajickova;Martin Haluzik
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
- Beta-2-microglobulin;
- Leptin;
- RNA extraction;
- RT-PCR;
2.Interactive Fluorophore and Quencher Pairs for Labeling Fluorescent Nucleic Acid Hybridization Probes

机译：相互作用的荧光团和猝灭剂对标记荧光核酸杂交探针。
- 作者：Salvatore A. E. Marras
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
3.PCR-Based Detection and Characterization of the Fungal Pathogens Colletotrichum gloeosporioides and Colletotrichum capsici Causing Anthracnose in Papaya (Carica papaya L.) in the Yucatan Peninsula

机译：基于PCR的尤卡坦半岛番木瓜炭疽病中炭疽菌和炭疽菌的真菌病原体检测和鉴定
- 作者：Raul Tapia-Tussell;Andres Quijano-Ramayo;Alberto Cortes-Velazquez;Patricia Lappe;Alfonso Larque-Saavedra;Daisy Perez-Brito
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
- Colletotrichum?capsici;
- Carica?papaya;
- Colletotrichum?gloeosporioides;
- PCR-RFLP;
- Restriction patterns;
- Sequencing of rDNA ITS region;
- Anthracnose;
4.Stem Cell Assays

机译：干细胞分析
- 作者：Gerald de Haan
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
5.Optimization of the AT-content of Codons Immediately Downstream of the Initiation Codon and Evaluation of Culture Conditions for High-level Expression of Recombinant Human G-CSF in Escherichia coli

机译：起始密码子下游紧邻的密码子AT含量的优化和在大肠杆菌中高效表达重组人G-CSF的培养条件的评估
- 作者：Dasari V. Krishna Rao;Joginapally V. Rao;Mangamoori L. Narasu;Adibhatla Kali S. Bhujanga Rao
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
- AT-rich codon;
- Codon optimization;
- High-level expression;
- Recombinant human G-CSF;
- Site-directed mutagenesis;
- Specific product yield;
6.Transcriptional Profiling of Gene Expression Changes in a PACE-Transfected CHO DUKX Cell Line Secreting High Levels of rhBMP-2

机译：转录分泌高水平的rhBMP-2的PACE转染CHO DUKX细胞系中基因表达变化的转录分析。
- 作者：Padraig Doolan;Mark Melville;Patrick Gammell;Martin Sinacore;Paula Meleady;Kevin McCarthy;Linda Francullo;Mark Leonard;Timothy Charlebois;Martin Clynes
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
7.Analysis of Context Sequence Surrounding Translation Initiation Site from Complete Genome of Model Plants

机译：从模型植物完整基因组分析翻译起始位点周围的上下文序列
- 作者：L. Rangan;C. Vogel;A. Srivastava
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
- A. thaliana;
- Consensus sequence;
- Eukaryotes;
- O. sativa;
- Translation initiation site;
8.An Efficient Method for the Extraction of High-Quality Fungal Total RNA to Study the Mycosphaerella fijiensis–Musa spp. Interaction

机译：一种高效的高质量真菌总RNA提取方法，用于研究斐济支原体–毛霉属。相互作用
- 作者：Aminael Sánchez-Rodríguez;Orelvis Portal;Luis E. Rojas;Bárbara Oca?a;Milady Mendoza;Mayra Acosta;Elio Jiménez;Monica H?fte
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
- cDNA libraries;
- Cladosporium?fulvum;
- Mycosphaerella?fijiensis;
- RNA isolation;
- Infected banana leaves;
9.Comparative Study of Bacillus thuringiensis Cry1Aa and Cry1Ac δ-Endotoxin Activation, Inactivation and In Situ Histopathological Effect in Ephestia kuehniella (Lepidoptera: Pyralidae)

机译：苏云金杆菌芽孢杆菌Cry1Aa和Cry1Acδ-内毒素的活化，失活和原位组织病理学作用的比较研究（鳞翅目：Pyralidae）
- 作者：Souad Rouis;Maissa Chakroun;Samir Jaoua
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
- Ephestia kuehniella;
- Bacillus thuringiensis;
- Proteolysis;
- Histopathological effect;
- Comparative study;
10.Indian Mustard Aquaporin Improves Drought and Heavy-metal Resistance in Tobacco

机译：印度芥菜水通道蛋白改善烟草的抗干旱和重金属能力
- 作者：Yuxiu Zhang;Zi Wang;Tuanyao Chai;Zhensong Wen;Hongmei Zhang
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
- Aquaporin;
- Drought;
- Heavy metal;
- Tobacco;
- Indian mustard;
11.Truncation Mutants Highlight a Critical Role for the N- and C-termini of the Spirulina Δ6 Desaturase in Determining Regioselectivity

机译：截短突变体突出了螺旋藻Δ6去饱和酶的N和C末端在确定区域选择性中的关键作用
- 作者：Pavinee Kurdrid;Matura Sirijuntarut;Sanjukta Subudhi;Supapon Cheevadhanarak;Apiradee Hongsthong
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
- Desaturase;
- Fatty acid desaturation;
- N-terminal truncation;
- C-terminal truncation;
- Spirulina platensis;
12.Development and Application of a Novel Signal Peptide Probe Vector with PGA as Reporter in Bacillus subtilis WB700: Twenty-Four Tat Pathway Signal Peptides from Bacillus subtilis were Monitored

机译：以PGA为报告基因的新型信号肽探针载体在枯草芽孢杆菌WB700中的开发和应用：监测枯草芽孢杆菌二十四种途径信号肽
- 作者：Fa-Ming Zhu;Sheng-Yue Ji;Wei-Wei Zhang;Wang Li;Bin-Yun Cao;Ming-Ming Yang
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
- PGA;
- B. subtilis WB700;
- Tat signal peptide;
- Secretion expression;
13.An Alternative Approach to Synthesize cDNA Bypassing Traditional Reverse Transcription

机译：绕过传统逆转录合成cDNA的另一种方法
- 作者：Xiao-Xia Li;Fang Zheng;Yan-Li Jiao;Gang Guo;Bao-Li Wang;Zhi Yao
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
- cDNA synthesis;
- DNA assembly;
- Class IIS restriction enzymes;
- Reverse transcription;
- Polymerase chain reaction;
14.MitoP2: An Integrative Tool for the Analysis of the Mitochondrial Proteome

机译：MitoP2：分析线粒体蛋白质组的综合工具
- 作者：Matthias Elstner;Christophe Andreoli;Uwe Ahting;Igor Tetko;Thomas Klopstock;Thomas Meitinger;Holger Prokisch
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
- Bioinformatics;
- In silico prediction;
- Mitochondrial database;
- Mitochondrial localization;
- Mitochondrial proteome;
- MitoP2;
- Proteomics;
15.PCR–SSCP: A Method for the Molecular Analysis of Genetic Diseases

机译：PCR–SSCP：遗传疾病分子分析的方法
- 作者：K. V. Kakavas;P. Plageras;A. T. Vlachos;A. Papaioannou;A. V. Noulas
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
16.The Genome Browser at UCSC for Locating Genes, and Much More!

机译：UCSC的基因组浏览器可用于定位基因，还有更多其他功能！
- 作者：Minou Bina
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
- The Human Genome Project;
- Genome browsers;
- Gene mapping;
- Gene display;
- Landmarks in genomic DNA;
17.A Method for Construction, Cloning and Expression of Intron-Less Gene from Unannotated Genomic DNA

机译：一种从未注释的基因组DNA缺失内含子基因的构建，克隆和表达方法
- 作者：Vineet Agrawal;Bharti Gupta;Uttam Chand Banerjee;Nilanjan Roy
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
- Exon–intron;
- Splicing;
- SOEP;
- MALDI;
- Aspergillus niger;
18.Rapid and Efficient Gene Splicing Using Megaprimer-Based Protocol

机译：使用基于Megaprimer的协议进行快速高效的基因剪接
- 作者：Ji-Ren Chen;Jing-Jing Lü;Hua-Fang Wang
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
- Gene splicing;
- Fusion PCR;
- Gene engineering;
- Megaprimer;
- Overlap extension;
19.cDNA-AFLP Analysis Reveals Differential Gene Expression in Response to Salt Stress in Foxtail Millet (Setaria italica L.)

机译：cDNA-AFLP分析揭示了谷子（Setaria italica L.）对盐胁迫的响应差异基因表达。
- 作者：Ananthi Jayaraman;Swati Puranik;Neeraj Kumar Rai;Sudhakar Vidapu;Pranav Pankaj Sahu;Charu Lata;Manoj Prasad
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
- Setaria italica;
- Salt stress;
- cDNA-AFLP;
- Transcript-derived fragments (TDFs);
- Quantitative real-time PCR (qRT-PCR);
- Gene expression;
20.Expression of Cholera Toxin B Subunit in Transgenic Rice Endosperm

机译：霍乱毒素B亚基在转基因水稻胚乳中的表达
- 作者：Maria Oszvald;Tae-Jin Kang;Sandor Tomoskozi;Barnabas Jenes;Tae-Geum Kim;Youn-Soo Cha;Laszlo Tamas;Moon-Sik Yang
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
- Cholera toxin B subunit;
- Rice;
- Endosperm-specific promoter;
- Plant-based vaccine;
21.Expression of Two Old Yellow Enzyme Homologues from Gluconobacter oxydans and Identification of their Citral Hydrogenation Abilities

机译：氧化葡糖杆菌中两个老黄色酶同系物的表达及其柠檬醛加氢能力的鉴定
- 作者：Bo Yin;Xuepeng Yang;Guodong Wei;Yushu Ma;Dongzhi Wei
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
- Gluconobacter n oxydans;
- Old yellow enzyme;
- Citral;
- NADH FMN-oxidoreductase;
- Citronellal;
22.Knockdown of Cellular RNA Helicase DDX3 by Short Hairpin RNAs Suppresses HIV-1 Viral Replication Without Inducing Apoptosis

机译：短发夹RNA敲低细胞RNA解旋酶DDX3抑制HIV-1病毒复制而不会引起细胞凋亡。
- 作者：Musarat Ishaq;Jiajie Hu;Xiaoyun Wu;Qiong Fu;Yalin Yang;Qingzhen Liu;Deyin Guo
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
- HIV-1;
- RNA helicase DDX3;
- RNAi;
- Short hairpin RNA;
- RNA nuclear export;
23.The Inhibitory Effect of an RGD-Human Chitin-Binding Domain Fusion Protein on the Adhesion of Fibroblasts to Reacetylated Chitosan Films

机译：RGD-人类几丁质结合域融合蛋白对成纤维细胞对乙酰化壳聚糖膜粘附的抑制作用。
- 作者：Vera Carvalho;Lucília Domingues;Miguel Gama
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
- Human chitin-binding module (ChBM);
- RGD;
- Fibroblasts;
- Cell adhesion;
- Reacetylated chitosan (RC) films;
24.Construction and Characterization of a Goat Mammary Gland cDNA Library

机译：山羊乳腺cDNA文库的构建与鉴定
- 作者：Xue Feng Han;Jun Luo;Ning Wu;Kanyand Matand;Bao Jin Yang;Hui Juan Wu;Li Juan Zhang;Hai Bin Wang
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
- cDNA library;
- Magnetic particle;
- Mammary gland;
25.Cloning and Sequencing of an Original Gene Encoding a Maltogenic Amylase from Bacillus sp. US149 Strain and Characterization of the Recombinant Activity

机译：编码芽孢杆菌麦芽淀粉酶原基因的克隆与序列分析US149菌株和重组活性的表征
- 作者：Sameh Ben Mabrouk;Ezzedine Ben Messaoud;Dorra Ayadi;Sonia Jemli;Amitava Roy;Monia Mezghani;Samir Bejar
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
- Cyclodextrinase;
- Inverse PCR;
- Kinetic parameters;
- Maltogenic amylase;
- Purification;
- Sequence homology;
26.Possible Role of Light and Polyamines in the Onset of Somatic Embryogenesis of Coffea canephora

机译：轻和多胺在小杯咖啡体胚发生中的可能作用
- 作者：Clelia De-la-Pe?a;Rosa M. Galaz-ávalos;Víctor M. Loyola-Vargas
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
- Coffea n canephora;
- Light;
- Polyamines;
- Somatic embryogenesis;
27.HBP21: A Novel Member of TPR Motif Family, as a Potential Chaperone of Heat Shock Protein 70 in Proliferative Vitreoretinopathy (PVR) and Breast Cancer

机译：HBP21：TPR母体家族的新成员，作为增生性玻璃体视网膜病变（PVR）和乳腺癌中热休克蛋白70的潜在伴侣
- 作者：Qinghuai Liu;Juanyu Gao;Xi Chen;Yuxin Chen;Jie Chen;Saiqun Wang;Jin Liu;Xiaoyi Liu;Jianmin Li
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
- HBP21;
- Heat-shock protein70;
- Tetratricopeptide repeats;
- Protein interaction;
28.The MicroRNA (miRNA): Overview of the RNA Genes that Modulate Gene Function

机译：MicroRNA（miRNA）：调节基因功能的RNA基因概述
- 作者：Shao-Yao Ying;Donald C. Chang;Shi-Lung Lin
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
- Small RNA;
- Non-coding RNAs;
- siRNA;
- miRNA;
- Intronic miRNA;
- Transposons;
- Biogenesis;
- Mechanism;
- Identification;
- Targeting;
- Fine-tuning;
- Gene function;
- Gene therapy;
- Anti-viral vaccine;
- Drug development;
- Future directions;
29.Reviews in Molecular Biology and Biotechnology: Transmembrane Signaling by G Protein-Coupled Receptors

机译：分子生物学与生物技术评论：G蛋白偶联受体的跨膜信号传导
- 作者：Louis M. Luttrell
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
30.An Epidermal Growth Factor-like Repeat of Del1 Protein Increases the Efficiency of Gene Transfer In Vitro

机译：Del1蛋白的表皮生长因子样重复增加体外基因转移的效率。
- 作者：Hisataka Kitano;Chiaki Hidai;Masatoshi Kawana;Shinichiro Kokubun
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
- Del1;
- Epidermal growth factor-like repeat;
- Gene transfer;
- Endocytosis;
31.High Frequency Plasmid Recombination Mediated by 28 bp Direct Repeats

机译：28 bp直接重复介导的高频质粒重组
- 作者：Sofia C. Ribeiro;Pedro H. Oliveira;Duarte M. F. Prazeres;Gabriel A. Monteiro
- 期刊名称：《Molecular Biotechnology》 | 2008年第3期
- Plasmid;
- Direct repeats;
- Deletion-duplication;
- Intramolecular recombination;
- Slipped misalignment;
- Escherichia?coli;

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