摘要:To understand how microbial communities and functional genes respond to arsenic contamination in the rhizosphere of Pteris vittata and in an arsenic contaminated soil from the surface to 4 m depth underground,surface soils and the 4 soil samples underground (1-4 m) were collected and investigated by Biolog,geochemical and functional gene microarray (GeoChip 3.0) analyses. Biolog analysis revealed that the uncontaminated soil harbored the greatest diversity of sole carbon utilization ability,and that arsenic-contamination decreased the metabolic diversity,while rhizosphere soils had higher metabolic diversities than the non-rhizosphere soils. GeoChip 3.0 analysis showed low proportions of overlapped genes across the five soil samples (16.52% - 45.75%).
摘要:A multiple metal(loid)-resistant strain Comamonas testosteroni S44 was isolated from mining waste soil. This bacterium exhibited a high Zn2+ resistant level with a minimal inhibitory concentration (MIC) of 10 mM. To understand the molecular basis for the high zinc resistance,whole genome sequencing was performed and showed large number of genes encoding putative metal(loid) resistance proteins,mobile genetic elements (MGEs),recent gene transposition,gene duplication and other events pointing to a mobile genome in the process of adaptation to a metal(loid)-contaminated environment. Particularly,a total of nine putative Zn2+ transporters (four putative zntA operons encoding P-type ATPase pumps and five czc operons encoding RND-transport efflux proteins) were identified in the draft genome sequence.
摘要:Arsenic(As) contamination in groundwater has become a major concern in many countries,the main source of arsenic is geological,but human activities such as mining and pesticides also cause arsenic pollution (Adsorption of arsenate and arsenite). Microorganisms are known to play an important role in the biochemical cycle of arsenic. Arsenic is a toxicmetalloid,which primarily exists in inorganic forms of arsenite [As(III)] and arsenate [As(V)].As(III) is much more toxic and mobile than As(V),hence As(III)-oxidizing bacteria with the ability to oxidize As(III) to much less hazardous As(V) provide great potentials for As(III)bioremediation. The most common As(III)-oxidizing bacteria reported so far were those from