High performance liquid chromatography–tandem mass spectrometry for rapid and sensitive analysis of six polycyclic aromatic hydrocarbons in wastewater

摘要

A sensitive and fast method was developed to quantitatively analyse the six polycyclic aromaticnhydrocarbons (fluoranthene (FLT), benzo[b]fluoranthene (BbF), benzo[k]fluoranthene (BkF), benzo[a]npyrene (Bap), benzo[ghi]perylene (BghiP) and indeno[1,2,3-cd]pyrene (INPY)) by high performancenliquid chromatography (UPLC) coupling with tandem mass spectrometry (MS/MS). Chromatographicnseparation was performed on a Waters Acquity UPLCTM BEHC18 column (1.7 μm, 2.1 mm× 50 mm).nA 0.2 μm precolumn filter was used to protect the analytical column. Mobile phase A was acetonitrilencontaining 0.5% toluene. Mobile phase B was water. Linearity of detection was in the range ofn1–100 μgLu00011n; LOD of 5 PAHs were lower than 0.1 μgLu00011n; LOQ were 0.2 μgLu00011nexcept fornbenzo[k]fluoranthene. The LOD and the LOQ of benzo[k]fluoranthene were respectively 0.1 μgLu00011nandn0.8 μgLu00011n. Wastewater samples collected from two wastewater treatment plants were determinednusing this method respectively. Recovery of all compounds varied from 67.8±10.6% to 113.2±n7.2%. In comparison with the existing methods, this rapid method saves time and solvent andnimproves instrument sample throughput by 2–5 fold.