Ecofriendly decolorisation of Cr-complex dye Acid Black 172 by a newly isolated Pseudomonas sp. strain DY1

摘要

Pseudomonas sp. strain DY1 was newly isolated from soil with rotten wood and identified as anmember of the genus Pseudomonas based on 16S rDNA and biochemical tests. Acid Black 172, anwater soluble Cr-complex dye, was then selected as a model dye to investigate the decolorisationnability of the strain. It was observed that the growth of the strain was not inhibited by high dosenof metal ions (10 mM), and efficient decolorisation was still observed when high concentrations ofnFe2t, Fe3t and Ca2t existed. The optimal decolorising conditions obtained from Taguchi designnwere as follows: temperature 371C, pH 7.0, Fe3t and proline concentrations of 7 mM and 3.0 g/L,nrespectively. Under the optimal conditions, 94.5% of Acid Black 172 (100 mg/L) could bendecolorised by the strain in 24 h. The kinetics of the decolorisation best fitted the first ordernkinetic model (R2紏0.981). Besides, the phytotoxicity study demonstrated a good detoxification bynthe strain. This work has a certain practical value in microbial decolorisation of textile wastewater.