Analysis of the bacterial community changes in soil for septic tank effluent treatment in response to bio-clogging

摘要

Soil columns were set up to survey the bacterial community in the soil for septic tank effluentntreatment. When bio-clogging occurred in the soil columns, the effluent from the columnsnwas in poorer quality. To evaluate changes of the soil bacterial community in response tonbio-clogging, the bacterial community was characterized by DNA gene sequences from soilnsamples after polymerase chain reaction coupled with denaturing gradient gel electrophoresisnprocess. Correspondence analysis showed that Proteobacteria related bacteria were the mainnbacteria within the soil when treating septic tank effluent. However, Betaproteobacteria relatednbacteria were the dominant microorganisms in the normal soil, whereas Alphaproteobacterianrelated bacteria were more abundant in the clogged soil. This study provided insight intonchanges of the soil bacterial community in response to bio-clogging. The results can supplynsome useful information for the design and management of soil infiltration systems.