Using combined measurements for comparison of light induction of stomatal conductance, electron transport rate and CO₂ fixation in woody and fern species adapted to different light regimes

摘要

We aimed to understand the relation of photosynthetic rate (A) with g_s and electron transport rate (ETR) in species of great taxonomic range and light adaptation capability during photosynthetic light induction. We studied three woody species (Alnus formosana, Ardisia crenata and Ardisia cornudentata) and four fern species (Pyrrosia lingus, Asplenium antiquum, Diplazium donianum and Archangiopteris somai) with different light adaptation capabilities. Pot-grown materials received 100 and/or 10% sunlight according to their light adaptation capabilities. At least 4 months after light acclimation, CO₂ and H₂O exchange and chlorophyll fluorescence were measured simultaneously by equipment in the laboratory. In plants adapted or acclimated to low light, dark-adapted leaves exposed to 500 or 2000 µmol m−2 s−1 photosynthetic photon flux (PPF) for 30 min showed low gross photosynthetic rate (P_g) and short time required to reach 90% of maximum P_g (). At the initiation of illumination, two broad-leaved understory shrubs and the four ferns, especially ferns adapted to heavy shade, showed higher stomatal conductance (g_s) than pioneer tree species; materials with higher g_s had short at both 500 and 2000 µmol m−2 s−1 PPF. With 500 or 2000 µmol m−2 s−1 PPF, the g_s for the three woody species increased from 2 to 30 min after the start of illumination, but little change in the g_s of the four ferns. Thus, P_g and g_s were not correlated for all material measured at the same PPF and induction time. However, P_g was positively correlated with ETR, even though CO₂ assimilation may be influenced by stomatal, biochemical and photoinhibitory limitations. In addition, was closely related to time required to reach 90% maximal ETR for all materials and with two levels of PPF combined. Thus, ETR is a good indicator for estimating the light induction of photosynthetic rate of species, across a wide taxonomic range and light adaptation and acclimation capability.