PECTOLYTIC ENZYMES PRODUCED BY THE PHYTOPATHOGENIC FUNGUS COLLETOTRICHUM GLOEOSPORIOIDES

摘要

The effect of different carbon sources on the production of extracellular pectolytic enzymes of Colletotrichum gloeosporioides was investigated. Production of polygalacturonase and pectinesterase was induced by pectin, sodium carboxymethyl cellulose, and xylan. Production of these enzymes was repressed or completely inhibited in the control cultures containing glucose as a carbon source and enzyme inducer. Maximum production of polygalacturonase and pectinesterase was induced when sodium carboxymethyl cellulose was used as the carbon source. The results indicate that the pectolytic enzymes of C. gloeosporioides may be produced in sequence, i.e., the production of polygalacturonase is followed by the secretion of pectinesterase.