SANGER WHO?

摘要

In November 2008 Elaine Mardis of Washington University in St. Louis and colleagues published the complete genome sequence of an individual with acute myeloid leukemia. Coming just a few years after the decade-long, muttibillion dollar Human Genome Project, the paper was remarkable on several levels. For one thing, the team sequenced two human genomes, both cancerous and normal, some 140 billion bases in all. More impressive, though, was what the study omitted: the 50 human genomes Mardis sequenced that year (albeit not as deeply) for the 1,000 Genomes Project. "It's like a whole new world," she says. Welcome to the sequencing frontier.rnElaine Mardis's acute myeloid leukemia work comprised about nine months of collecting 32-base snippets at the rate of about a billion bases per instrument every five days, with five instruments running in parallel, she says. "That seemed like a huge amount [of sequence] at the time," she recalls.