DNA SAMPLE PREPARATION

摘要

PureGenome kits and reagents are designed for rapid and efficient next generation sequencing (NGS) sample preparation. With these reagent sets, library preparation has been streamlined to two steps in under two hours, followed by a short enrichment step, thus alleviating a typical bottleneck in the sequencing process. PureGenome library preparation is a simple, two-step process followed by amplification using EMD Millipore's ultrahigh fidelity KOD Hot Start DNA Polymerase Mastermix. This unique polymerase amplifies DNA with high processivity in highly thymine-adenine (TA)- or guanine-cytosine (GC)-rich regions.