Introduction of a rice blight resistance gene, Xa21, into five Chines e rice varieties through an Agrobacterium-mediated System

摘要

a cloned gene, Xa21 was transferred into five widely-used Chinese rice varieties through an Agrobacterium-Mediated system, and over 110 independent transgenic lines were ob- tained. PCR and Southern analysis of transgenic plants revealed the integration of the whole xa21 gene into the host genomes. The integrated xa21 gene was stably inherited, and segregated ina 3:1 ratio in the selfed T_1 generation when one copy of the gene was integrated in the transfo- mants.