Detection of the variant frequency in human erythrocytes using glycophorin a locus mutation assay

摘要

Glycophorin A (GPA) is the major glycoprotein on human erythrocyte membranes, and bears M and N blood group determinants. Genotoxic exposure causes erythrodi precursor cells to mutate at the glycophorin. A locus, producing phenotypes of are variant erythrocytes that lack the expression of one form of the protein. The BR6 assay is now the most widely used method to determine the variant frequency (Vf) by immunolabelling and flow cytometry. In this study, two kinds of labelling were used.