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Isolation and Identification of Two Membrane Proteins Binding to Transfer RNA

机译:结合转移RNA的两种膜蛋白的分离和鉴定

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摘要

Two major tRNA binding proteins were obtained by biochemical method from yeast crude extracts of total membrane proteins. They were further confirmed to be tRNA specific-binding proteins by gel mobility shift assay. N-terminal sequence assays showed that they were glyceraldehyde-3-phosphate dehydranase (GAPDH) and 3-phosphate glycerate kinase (PGK1), respectively.
机译:通过生化方法从总膜蛋白的酵母粗提取物中获得了两种主要的tRNA结合蛋白。通过凝胶迁移率移动测定进一步证实它们是tRNA特异性结合蛋白。 N末端序列分析表明它们分别是3-磷酸甘油醛脱水酶(GAPDH)和3-磷酸甘油酸酯激酶(PGK1)。

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